Zinc finger nucleases (ZFNs) enable manipulation of the genome with unprecedented ease and precision. ZFNs are a class of engineered DNA-binding proteins that facilitate targeted editing of the genome by creating double-strand breaks in DNA at user-specified locations. ZFNs have already been used to generate targeted genomic deletions of large segments of DNA in different cell types and organisms like mouse and human cells, plants, fruit flies, zebrafish, and rat. Recently, ZFNs have been used to disrupt or tag genes in human pluripotent stem cells, a system of tremendous interest for genome engineering that was lacking a reliable gene manipulation tool. Featuring keynote presentations, this conference will allow participants to discover the latest applications of transgenics and cell based models for the study of gene regulation and disease. Comprehensive lectures provide in-depth discussion on the fundamentals of ZFNs as well as innovative applications for targeted gene knockout, integration, and tagging. Geared towards scientists interested in learning about the technology, the conference provides researchers at all levels with a unique opportunity for networking with peers. Discussions will facilitate the exchange of ideas to shape the future directions of research in this key area.