With the continuous expansion of sturgeon aquaculture worldwide, the widespread circulation of various breeds and hybrid individuals has led to germplasm mixing, affecting product quality and traceability. There is an urgent need for rapid, accurate, and cost-effective identification technologies. Based on whole-genome resequencing data from several sturgeon species, this study screened species-specific locus and developed corresponding molecular markers. Through the design and validation of PCR primers, the specificity and universality of these markers were verified in several major cultured sturgeon species in China. The dominant and co-dominant molecular markers developed by this method have been validated in a large number of purebred and hybrid sturgeon, yielding accurate and reliable results. Compared with traditional microsatellite marker methods, the detection period was reduced from 10 days to 4 hours. The speices identification method based on sturgeon SNP/InDel molecular markers mined from resequencing data offers high sensitivity, high throughput, and low cost. It meets the needs of aqua-farms for rapid identification, superior breed promotion, and product traceability, and holds significant value for the sustainable development of the sturgeon industry and the conservation of genetic resources.
 

Sturgeon,species Identification,Whole Genome Resequencing,Molecular Marker