43 / 2024-10-18 17:35:49

Detection of swinepox virus antibodies and characterization of their linear B-cell epitopes by an efficient blocking ELISA based on p35 monoclonal antibody

Members of the Poxviridae family cause a wide range of poxvirus-associated diseases in humans and animals, including cowpox virus, sheep pox virus, swinepox virus(SWPV), monkeypox virus, pigeonpox virus, and many more. In 2022, an outbreak of monkeypox occurred in Europe and rapidly spread around the globe, with multiple fatalities reported, and it still has far-reaching effects around the world today. More than that, it has been reported that some poxviruses can infect unnatural hosts, which suggests the cross-species transmissibility of these viruses, and it is necessary to investigate whether Swinepox Virus, which is a member of the same family, could be on an epidemic trend.
Swinepox is an acute, often mild, infectious disease characterized by typical pox lesions in the skin and affects only pigs. The causative agent for swinepox is swinepox virus (SWPV), the sole member of the genus suipoxvirus, belonging to the sub-family chordopoxrinae under family Poxviridae. It is an enveloped virus with typical poxviral brick-shaped morphology and a large linear double-stranded DNA genome with a cytoplasmic site of replication
There is no report on the study of the function of p35 protein of swinepox virus, but poxviruses, as the prototype virus of poxviruses, have been studied more clearly! Poxvirus p35 protein is an immunodominant antigenic protein on the surface of the vesicle membrane of intracellular mature virus particles , and it is the main protein that induces the production of antibodies, especially in the generation of the second immune response, and is involved in viral adsorption to host cells, and has its major roles in the binding and assembly of mature virus particles, viral virulence and immunogenicity.
in this study, an anti-p35 mAb against SWPV p35 was prepared and an efficient blocking ELISA was established. The new assay may serve as an important tool for serologic surveillance of SWPV infection. In addition, a linear B-cell epitope region located between 114 KDPEKK 119 and 23 QDVIPDI 29 on p35 was identified. In addition to its important role in the serologic diagnosis of SWPV, the authors' findings provide a solid basis for further investigation of the antigenic function of p35