41 / 2024-10-02 13:43:30

单细胞尺度空间Hi-C技术探究哺乳动物脑的染色质高级结构

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The 3D chromatin architecture is crucial for understanding gene regulatory programs in the development and disease. However, the direct mapping genome-wide 3D chromatin structures while retaining spatial information in tissues is still lacking. Here, we develop a spatial Hi-C method to resolve chromatin architecture profiling in situ tissue at single-cell resolution. The spatial Hi-C alone can obviously distinguish different cell niches in mammalian brains. Single-cell spatial Hi-C profiles in mouse brains show a decreased trend of the long-range interactions along the inside-to-outside radial direction on coronal brain sections, which is associated with neuronal differentiation states. In all, spatial Hi-C provides a valuable tool to study spatial 3D genome regulation in complex tissues.The 3D chromatin architecture is crucial for understanding gene regulatory programs in the development and disease. However, the direct mapping genome-wide 3D chromatin structures while retaining spatial information in tissues is still lacking. Here, we develop a spatial Hi-C method to resolve chromatin architecture profiling in situ tissue at single-cell resolution. The spatial Hi-C alone can obviously distinguish different cell niches in mammalian brains. Single-cell spatial Hi-C profiles in mouse brains show a decreased trend of the long-range interactions along the inside-to-outside radial direction on coronal brain sections, which is associated with neuronal differentiation states. In all, spatial Hi-C provides a valuable tool to study spatial 3D genome regulation in complex tissues.