Using chemical methods to load TiO2 on the glass material is beneficial to the addition and recovery of TiO2.The results demonstrated that the addition of TiO2 loaded glass materials could enhance the photosynthetic biohydrogen production, with the best effect of glass beads loading on hydrogen production, which increased by 13% compared to the control group. The change in reducing sugar concentration indicated that the addition of titanium dioxide could encourage the photosynthetic bacteria to make more use of sugar for hydrogen production. The experimental results show that the addition of TiO2 loaded glass is beneficial to the hydrogen production process of photosynthetic biohydrogen production, which can provide scientific reference for the further optimization and industrial development of photosynthetic biohydrogen production technology.Using chemical methods to load TiO2 on the glass material is beneficial to the addition and recovery of TiO2.The results demonstrated that the addition of TiO2 loaded glass materials could enhance the photosynthetic biohydrogen production, with the best effect of glass beads loading on hydrogen production, which increased by 13% compared to the control group. The change in reducing sugar concentration indicated that the addition of titanium dioxide could encourage the photosynthetic bacteria to make more use of sugar for hydrogen production. The experimental results show that the addition of TiO2 loaded glass is beneficial to the hydrogen production process of photosynthetic biohydrogen production, which can provide scientific reference for the further optimization and industrial development of photosynthetic biohydrogen production technology.