In order to explore the effect of antibiotic fermentation dregs biochar on anaerobic methanogenesis of food waste. The inoculum to substrate ratio (ISR) was set at 1.5 for batch anaerobic digestion. The pH of the system and the dosage of biochar were fixed. The blank group and biochar experimental group were set. The surface morphology, elements, functional groups, potential and pH of the two biochars were characterized, and the daily methane production, pH, volatile fatty acids (VFAs) and bicarbonate alkalinity (BA) of the anaerobic digestion system were continuously monitored. All biochars were alkaline and had the ability to adsorb positive ions; The addition of spectinomycin fermentation dregs biochar (DGBC) increased the total methane production by 6.24%, and the pH of anaerobic digestion system was always higher than that of blank group, which was about 7.5; In terms of total gas production, DGBC increased by 5.3%, TLBC decreased by 0.84%, and YMBC increased by 15.38%. The addition of DGBC and YMBC made the system have better buffer capacity. Meanwhile, in terms of microbial diversity, the addition of TLBC inhibited the abundance of Clostridium and methanothrix, resulting in the decrease of cumulative methane production. Therefore, the methane production performance of the anaerobic digestion system of food waste can be improved by adding spectinomycin fermentation dregs biochar, which provides a more meaningful path for the treatment of spectinomycin fermentation dregs