116 / 2021-06-30 23:33:06

Effect of α-amylase on biological hydrogen production by photo-fermentation of moldy wheat

moldy wheat,optimal enzyme load,substrate concentration,specific hydrogen production rate,pH value

Biological hydrogen production from mildewy wheat is a practical treatment strategy, because part of the starch in wheat has been decomposed into organic acids, which can reduce the energy consumption of bacteria during hydrogen production, and can be quickly decomposed and utilized under the catalysis of amylase.In this experiment, HAU-M1 was used as the photofermentation bacteria, the slightly moldy wheat was selected as the substrate, and α-amylase was used as the catalyst. 0.1 g,0.2 g,0.3 g,0.4 g,0.5 g α-amylase and 1 g,3 g,5 g,7 g,9 g substrate were added respectively to study the effect of enzyme amount and substrate concentration on the hydrogen production performance of moldy wheat.Hydrogen production potential of mildewed wheat was investigated.The results showed that the increase of enzyme content could improve the hydrogen production rate, but it was not conducive to the increase of cumulative hydrogen production when the enzyme load was 0.06 g/gTS. When the enzyme load was 0.06 g/gTS, the comprehensive effects of hydrogen production rate and cumulative hydrogen production were the best, which were 27.5  mL /h and 122.8  mL H2/gTS, respectively, which were increased by 76.2% and 5.1% compared with the control group.When the substrate concentration was 3 g, the maximum specific hydrogen production rate was 172 mlH2/gTS.