摘要详情
195 / 2024-07-09 11:56:58
Detection of cuproptosis microenvironment based on fluorescence lifetime imaging
cuproptosis,fluorescence lifetime
全文待审
Detection of cuproptosis microenvironment based on fluorescence lifetime imaging
Yixiong Su
College of Optoelectronic Engineering, Shenzhen University, Shenzhen, Guangdong 518060, P.R. China.
2210452015@email.szu.edu.cn
Abstract
The steady-state imbalance of copper ions can lead to an increase in copper ion concentration in the body, causing cell damage and cuproptosis. Copper ions catalyze hydrogen peroxide to produce a large amount of reactive oxygen species, resulting in lipid peroxidation. Lipid peroxidation significantly changes the composition and structure of the membrane, leading to changes in polarity. The polarity changes in different intracellular regions have been recognized as reliable indicators of different diseases, monitoring the polarity of cell membranes and intracellular lipid droplets is of great significance as a diagnostic and research indicator of cuproptosis. Using probes to stain cells and analyzing changes in subcellular organelle microenvironment during cuproptosis using fluorescence lifetime imaging. Establishing a copper induced cell death model through copper death inducers, and real-time monitoring of changes in lipid droplets and cell membrane polarity during cuproptosis process. It was found that under the action of Elesclomol, the lipid droplet content in cells increased significantly, and the fluorescence lifetime of the probe decreased significantly, indicating that the polarity of intracellular lipid droplets increased.
Keywords: cuproptosis, cell microenvironment, fluorescence lifetime imaging, polarity, lipid, cell membrane
重要日期
-
会议日期
09月08日
2024
至09月12日
2024
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09月15日 2024
初稿截稿日期
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09月15日 2024
注册截止日期
主办单位
ShenZhen University
联系方式
- Organizing Committee
- ic******@163.com
- +86********
历届会议
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2009年05月11日 中国 哈尔滨市
2009年纳米光子学国际会议
