摘要详情
162 / 2024-06-11 18:49:34
Highly-Sensitive Detection and Quantification of Amyloid-β Aggregation in Alzheimer's Disease via Fluorescence Lifetime Imaging
Fluorescence Lifetime Imaging, Alzheimer's disease, Amyloid-beta, photobiomodulation
全文待审
Accurate detection and quantification of Amyloid-beta (Aβ) aggregation are crucial for understanding the mechanisms underlying Alzheimer's disease (AD). Despite advancements, conventional fluorescence imaging techniques still struggle to precisely monitor specific aggregation states. In this study, we developed NIR-Aβ, a polarity-sensitive fluorescent lifetime probe, to monitor Aβ protein aggregation dynamics during AD progression. Both in vitro and in vivo experiments confirm NIR-Aβ's Aβ-specific targeting, high polarity detection sensitivity, and excellent blood-brain barrier penetration. Using fluorescence lifetime imaging (FLIM) with NIR-Aβ, we successfully tracked Aβ aggregation dynamics in photobiomodulation experiments. Interestingly, we observed significant changes in Aβ aggregation fluorescence lifetime following photobiomodulation treatment, despite unchanged morphology, decreasing from 1.5 ns to 0.8 ns. These findings pave the way for accurately assessing and quantifying photobiomodulation therapy's impact on AD using FLIM, enhancing therapeutic evaluation and clinical outcome prediction.
重要日期
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会议日期
09月08日
2024
至09月12日
2024
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09月15日 2024
初稿截稿日期
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09月15日 2024
注册截止日期
主办单位
ShenZhen University
联系方式
- Organizing Committee
- ic******@163.com
- +86********
历届会议
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2009年05月11日 中国 哈尔滨市
2009年纳米光子学国际会议
